《植物生理学报》 2009, 45(7): 651-656

野葛葡糖基转移酶基因PlUGT3 的克隆与生物信息学分析

周文灵¹, 王瑛华^1,2, 陈刚², 李玲^1,*

1 华南师范大学生命科学学院广东省植物发育生物工程重点实验室, 广州510631; 2 肇庆学院生物系, 广东肇庆526061

通信作者：李玲；E-mail: lilab@scnu.edu.cn；Tel: 020-85211378

摘　要：

采用 3' 和 5' RACE 技术从野葛中克隆到葡糖基转移酶基因 PlUGT3 (GenBank 登录号 EU889121) cDNA 序列, 长度为1678 bp, 包含完整的开放阅读框(ORF)。ORF 全长 1 428 bp, 编码 475 个氨基酸。此氨基酸序列具有葡糖基转移酶糖基受 体结合特征区域 PSPG box, 与几种高等植物氨基酸序列的同源性大于 40%。半定量 RT-PCR 表明, PlUGT3 在野葛根和叶 中表达, 两者表达量没有明显差别。

关键词：野葛; 葡糖基转移酶; RACE; 生物信息学分析

收稿：2009-03-10   修定：2009-05-07

资助：广东省高等院校专科建设专项资金(LYM08102)和广东省自然科学基金(04010377)。

Cloning and Bioinformatic Analysis of Glucosyltransferases Gene PlUGT3 in Pueraria lobata (Willd.) Ohwi

ZHOU Wen-Ling¹, WANG Ying-Hua^{1, 2}, CHEN Gang², LI Ling^1,*

¹Guangdong Key Laboratory of Biotechnology for Plant Development, College of Life Science, South China Normal University, Guangzhou 510631, China; ²Department of Biology, Zhaoqing University, Zhaoqing, Guangdong 526061, China

Corresponding author: LI Ling; E-mail: lilab@scnu.edu.cn; Tel: 020-85211378

Abstract:

A glucosyltransferase gene, named PlUGT3 (GenBank accession No. EU889121) was cloned from Pueraria lobata by RT-PCR and RACE methods. The cDNA contained 1 678 bp with an open reading frame (ORF) of 1 428 bp and encoded a putative protein of 457 amino acids. The deduced amino acid sequence showed more than 40% similarity to homologues from other plants such as Rhodiola sachalinensis and Arabidopsis thaliana, and to highly conserved functional domain PSPG box of the gene family. Semiquantitive RT-PCR analysis indicated that PlUGT3 expression levels were not significant different in roots and leaves.

Key words: Pueraria lobata; glucosyltransferases; RACE; bioinformatic analysis